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產(chǎn)品名稱：干細胞生長分化因子
干細胞生長分化因子的產(chǎn)品信息
Wnt糖蛋白是存在于多種生物體內(nèi)的一種細胞外配體，Wnt作為形態(tài)發(fā)生素通過激發(fā)細胞內(nèi)遠離信號發(fā)送區(qū)域的濃度依賴反應(yīng)控制胚胎形態(tài)發(fā)育。Wnt通路調(diào)節(jié)動物發(fā)育過程中的多個重要環(huán)節(jié)，例如細胞增殖、細胞遷徙及細胞分化。Wnt蛋白通過自分泌或旁分泌作用與位于細胞膜上的受體相結(jié)合，激活細胞內(nèi)信號通路調(diào)節(jié)靶基因的表達，對細胞的增殖、分化、遷移、極性化和凋亡起到重要作用。
WNT-5a,human W5A-H-005,5ug 產(chǎn)品簡介
Wnt信號轉(zhuǎn)導通路因其啟動蛋白為Wnt蛋白而得名，在胚胎發(fā)育、細胞增殖及分化等方面發(fā)揮重要 作用，其異常活化與人類多種腫瘤的發(fā)生密切相關(guān)。研究顯示，Wnt信號至少存在經(jīng)典和非經(jīng)典兩條不同的信號通路，而Wnt-5a屬于非經(jīng)典Wnt信號通 路，既往研究Ror2具有Wnt受體結(jié)構(gòu)域樣結(jié)構(gòu)。有研究認為Wnt-5a經(jīng)由Ror2介導的 信號通路發(fā)揮作用。
Human WNT-5a蛋白含有357個氨基酸殘基。
人源WNT-5a 蛋白因子，5ug 產(chǎn)品特性
? 活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定，純度高，無動物成分污染
?人源WNT-5a 蛋白因子，5ug 活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細胞鑒定
? 人源WNT-5a 蛋白因子，5ug 產(chǎn)品經(jīng)過SDS-PAGE 和HPLC雙層鑒定純度大于85%
人源WNT-5a 蛋白因子，5ug 保存方法
The lyophilized protein is stable for at least 6 months if stored at -80 degreeC. Reconstituted protein is stable for at least two weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid repeated freeze and thaw.
產(chǎn)品英文描述
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical” pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product WNT-5a,human W5A-H-005,5ug is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
?WNT-5a,human W5A-H-005,5ug Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
?WNT-5a,human W5A-H-005,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparat
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